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目的对蔷薇科植物月季花的化学成分进行分离和鉴定。方法本实验采用硅胶柱色谱、聚酰胺柱色谱,SephadexLH-20柱色谱和重结晶等分离纯化手段对化合物进行纯化,并根据波谱分析技术和理化性质对照等方法对化合物进行结构鉴定。结果从其石油醚和三氯甲烷部位分离得到了15个化合物,分别为:β-谷甾醇(1)、菜油甾醇(2)、环桉烯醇(3)、齐墩果酸(4)、熊果酸(5)、2α,3α,19α,23-四羟基-12-烯-28-乌苏酸(6)、2α,3α,19α,-三羟基乌苏-12-烯-28-酸(7)、山柰酚(8)、槲皮素(9)、山柰酚-3-O-β-D-葡萄糖苷(10)、山柰酚-3-O-β-D-槐糖苷(11)、槲皮素-3-O-β-D-槐糖苷(12)、槲皮素7-O-β-龙胆二糖苷(13)、银椴苷(14)、芹菜素-7-O-(6″-O-对羟基苯甲酰基)-β-D-葡萄糖苷(15)。结论其中化合物2,4,5,6,7,12,13,14,15等9个化合物,为首次从月季植物中分离得到。
Aim To isolate and identify the chemical constituents of the rose flower of the Rosaceae. Methods The compounds were purified by silica gel column chromatography, polyamide column chromatography, Sephadex LH-20 column chromatography and recrystallization. The structures of the compounds were identified by spectroscopic analysis, physical and chemical properties and other methods. Results Fifteen compounds were isolated from the petroleum ether and trichloromethane fractions, which were: β-sitosterol (1), campesterol (2), cyclogallinol (3), oleanolic acid Ursolic acid (5), 2α, 3α, 19α, 23-tetrahydroxy-12-ene-28-ursuic acid (6), 2α, 3α, 19α-trihydroxysussu-12-en-28- (7), kaempferol (8), quercetin (9), kaempferol-3-O-β-D-glucoside (12), quercetin 7-O-β-gentiobioside (13), tylosin (14), apigenin-7 -O- (6 "-O-p-hydroxybenzoyl) -β-D-glucoside (15) .Conclusion Among them, 9 compounds such as 2,4,5,6,7,12,13,14,15 , For the first time isolated from the rose plant.