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目的研究人参皂苷Rh2、细胞因子诱导的杀伤(CIK)细胞及二者联合对胃癌细胞株SGC-7901的体外增殖抑制作用。方法本试验采用MTT法检测30、35、40及45μg/ml人参皂苷Rh2以及效靶比分别为5:1、10:1、20:1和40:1的CIK细胞,35μg/ml的人参皂苷Rh2联合效靶比20:1的CIK细胞,分别作用于SGC-7901细胞24 h和48 h的增殖抑制作用。结果不同剂量的人参皂苷Rh2作用于SGC-7901细胞,在相同作用时间内,抑制率随着剂量的增加而增加,差异有统计学意义(P<0.01);随给药时间的延长,除45μg/ml组外,其他各剂量组作用48 h的抑制率均较24 h有显著上升,差异有统计学意义(P<0.05或0.01)。不同效靶比的CIK细胞作用于SGC-7901细胞后,在相同作用时间内,抑制率随着效靶比的增加而增加,差异有统计学意义(P<0.01);相同效靶比条件下,抑制率随着作用时间的延长而增加,差异均具有统计学意义(P<0.01)。35μg/ml的人参皂苷Rh2联合20:1的CIK细胞对SGC-7901细胞的增殖抑制作用显著强于人参皂苷Rh2或CIK细胞单用,差异均具有统计学意义(P<0.05或0.01)。结论人参皂苷Rh2及CIK细胞在体外对胃癌细胞株SGC-7901均具有一定增殖抑制作用,且在一定的条件下二者联合应用具有协同效果,较单用具有更强的抑制SGC-7901细胞增殖的作用。
Objective To study the inhibitory effects of ginsenoside Rh2, cytokine-induced killer (CIK) cells and their combination on the proliferation of gastric cancer cell line SGC-7901 in vitro. Methods In this study, 30,35,40 and 45μg / ml ginsenoside Rh2 and CIK cells with 5: 1, 10: 1, 20: 1 and 40: 1 effective target ratios were detected by MTT assay, 35μg / ml ginsenoside The CIK cells with Rh2 combined effect target ratio 20: 1 were respectively used to inhibit the proliferation of SGC-7901 cells 24 and 48 h. Results Different dosages of ginsenoside Rh2 in SGC-7901 cells in the same duration of time, inhibition rate increased with the dose increased, the difference was statistically significant (P <0.01); with the extension of time, except 45μg / ml group, the other 48 h dose rate of each group were significantly higher than the inhibitory rate of 24 h, the difference was statistically significant (P <0.05 or 0.01). After CIK cells with different effective target ratios were treated with SGC-7901 cells, the inhibitory rate increased with the increase of the ratio of target to target in CIK cells with the same effect target (P <0.01) , The inhibition rate increased with the extension of time, the differences were statistically significant (P <0.01). The inhibitory effect of 35μg / ml ginsenoside Rh2 combined with 20: 1 CIK cells on SGC-7901 cell proliferation was significantly stronger than that of ginsenoside Rh2 or CIK cells alone, the difference was statistically significant (P <0.05 or 0.01). Conclusion Ginsenoside Rh2 and CIK cells can inhibit proliferation of gastric cancer cell line SGC-7901 in vitro. Under certain conditions, synergistic effect of the combination of the two has been found. Compared with single use, Ginsenoside Rh2 and CIK cells have stronger inhibitory effect on proliferation of SGC-7901 cells Role.