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观察0号柴油水溶性成分(DWSF)对褐菖鲉(Sebastiscus marmoratus)肝脏、脾脏和肾脏抗氧化能力的影响.实验条件下,褐菖鲉通过水体分别暴露于0.02,0.1,0.5mg/L的0号柴油水溶性成分,于暴露后0,7,28,84d和恢复实验的7,28d采样进行理化分析.MDA含量在肝脏组织中高质量浓度组暴露期间表现出显著性降低,脾脏中暴露初期(7,28d)有显著或极其显著抑制现象,肾脏在胁迫过程中表现为低质量浓度组降低,高质量浓度组先降低再升高后恢复;GPx活性表现肝脏为先诱导再抑制后诱导,脾脏为先诱导后抑制,肾脏则表现被抑制;比较3器官的抗氧化指数变化,抗氧化防御系统对DWSF的抗氧化能力依次表现为肝脏>肾脏>脾脏,其中对污染物敏感性最高的是脾脏.
The effects of DWSF on the antioxidant capacity of liver, spleen and kidney in Sebastiscus marmoratus were observed under the experimental conditions, 0 diesel fuel water-soluble components, at 0, 7, 28, 84d after exposure and recovery experiments 7,28d sampling for physical and chemical analysis.MDA content in the liver tissue of high-quality concentration group showed significant decrease during exposure, early spleen exposure (7,28 days) showed significant or extremely significant inhibition. The kidney showed a decrease in low-quality concentration group during stress and a decrease in high-quality concentration group and then increased again. GPx activity showed that the liver was induced first and then suppressed, The spleen was induced first and then inhibited, and the kidney was inhibited. Comparing the change of antioxidant index of three organs, the anti-oxidative defense system’s ability of anti-oxidation against DWSF turned out to be liver> kidney> spleen, of which the highest sensitivity to pollutants spleen.