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近几年,免疫组化染色在病理诊断和科研工作中应用颇为广泛。免疫组化染色方法的特异性较高,而且近年多克隆抗体和单克隆抗体的应用种类越来越多,用于回顾性的诊断或研究也逐步开展。在实际工作中,常因原始蜡块丢失,或组织块的病变很小在后续切片中病变区消失,或仅保留一张切片(如脱落细胞涂片)等原因而不能重新制片。原有的HE染色切片能否再作免疫组化染色,成为一个需要探讨的问题。有人已做过这方面尝试,但仅用一种单克隆抗体。为此我们在HE染色切片(或涂片)上进行17种多克隆抗体和8种单克隆抗体的免疫组化染色,获得一些初步体会。
In recent years, immunohistochemical staining in the pathological diagnosis and research work is quite extensive. Immunohistochemical staining method has higher specificity, and more and more types of polyclonal antibodies and monoclonal antibodies are used in recent years, which are used for retrospective diagnosis or research. In practice, often the original wax block is lost, or the lesion of the tissue block is small in the subsequent section of the lesions disappear, or only retain a slice (such as exfoliated cells smear) and other reasons can not be re-made. The original HE stained sections can be re-immunohistochemical staining, become a need to explore the issue. Someone has done this attempt, but only a single monoclonal antibody. To this end, we performed immunohistochemical staining of 17 kinds of polyclonal antibodies and 8 kinds of monoclonal antibodies on HE-stained sections (or smears), and obtained some preliminary experiences.