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目的：探讨鼻咽癌中ｎｍ２３Ｈ１ｍＲＮＡ和蛋白的表达及临床意义。方法：收集７８例低分化鼻咽癌初治患者新鲜鼻咽部活检标本，用原位杂交检测ｎｍ２３Ｈ１的ｍＲＮＡ表达并用免疫组化检测其蛋白质表达。结果：伴有淋巴结转移的５３例标本中，ｎｍ２３Ｈ１ｍＲＮＡ和蛋白的表达率分别为４０％和３６％；而２５例无淋巴结转移的两者表达率均为９６％。ｎｍ２３Ｈ１阴性表达组的淋巴结转移率显著高于阳性表达组（Ｐ＜０．００１）。结论：①ｎｍ２３Ｈ１基因ｍＲＮＡ和蛋白质的表达与鼻咽癌淋巴结转移潜能呈负相关。②鼻咽癌中ｎｍ２３Ｈ１在ｍＲＮＡ及蛋白层次的表达基本一致。结果提示ｎｍ２３Ｈ１基因的表达在抑制鼻咽癌转移过程中起着一定的作用。
Objective: To investigate the expression of nm23H1 mRNA and protein in nasopharyngeal carcinoma and its clinical significance. Methods: Fresh nasopharyngeal biopsies were collected from 78 newly diagnosed patients with poorly-differentiated nasopharyngeal carcinoma. The expression of nm23-H1 mRNA was detected by in situ hybridization and the protein expression was detected by immunohistochemistry. Results: In 53 specimens with lymph node metastasis, the expression rates of nm23-H1 mRNA and protein were 40% and 36%, respectively. However, the expression rates of both 25 cases without lymph node metastasis were 96%. The rate of lymph node metastasis in nm23 H1 negative group was significantly higher than that in positive group (P <0.001). Conclusion: ① The expression of nm23 H1 gene mRNA and protein is negatively correlated with the lymph node metastasis potential of nasopharyngeal carcinoma. ② nm23 Hlopharyngeal carcinoma in the expression of mRNA and protein levels are basically the same. The results suggest that nm23 H1 gene expression in the inhibition of nasopharyngeal carcinoma metastasis plays a role.