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目的 :报道ｐ62 ｄｏｋ蛋白的纯化和氨基酸及ｃＤＮＡ序列的测定方法和结果 ,拟为研究ｐ62 ｄｏｋ在胰岛素信息传递中的作用提供必需的技术方法和试剂。方法 :过渡表达人类胰岛素受体的中国仓鼠卵巢细胞(ＣＨＯ/ＩＲ)细胞用含 1 0 %胎牛血清的ＨａｍｓＦ - 1 2培养 1 2ｈ( 37℃
OBJECTIVE: To report the purification of p62 dok protein and determination of amino acid and cDNA sequence, and to provide the necessary technical methods and reagents for studying the role of p62 dok in insulin signaling. METHODS: Chinese Hamster Ovary cells (CHO / IR) cells transiently expressing human insulin receptor were cultured with HamsF - 1 2 containing 10% fetal calf serum for 12 h