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目的:探讨失血性休克大鼠血清对人脐静脉内皮细胞内皮细胞蛋白C受体(EPCR)表达的影响。方法:采用酶联免疫吸附试验(ELISA)、逆转录聚合酶链反应(RT-PCR)、WESTERN BLOT技术分别对正常对照组、失血性休克(3h、12h、24h、72h)组大鼠血清培养的人脐静脉内皮细胞(HUVEC)可溶性EPCR(sEPCR)水平和EPCR mRNA及蛋白表达进行检测。结果:与正常对照组比较,休克3h、72h组sEPCR、EPCR的mRNA、蛋白含量均无明显变化(P均>0.05),休克12h、24h组sEPCR、EPCR的mRNA含量均显著增高(P均<0.05),EPCR的蛋白含量均显著降低(P均<0.05)。结论:失血性休克大鼠血清可以从基因、蛋白水平影响人脐静脉内皮细胞EPCR的表达。
Objective: To investigate the effect of hemorrhagic shock rat serum on the expression of endothelial cell protein C receptor (EPCR) in human umbilical vein endothelial cells. Methods: Serum culture of rats in normal control group, hemorrhagic shock group (3h, 12h, 24h, 72h) was performed by enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR) and Western blotting Of human umbilical vein endothelial cells (HUVEC) soluble EPCR (sEPCR) levels and EPCR mRNA and protein expression were detected. Results: Compared with the normal control group, the mRNA and protein levels of sEPCR and EPCR did not change significantly at 3h and 72h after shock (all P> 0.05). The mRNA and protein expressions of sEPCR and EPCR increased significantly at 12h and 24h after shock (P < 0.05), EPCR protein content was significantly lower (P all <0.05). Conclusion: The serum of hemorrhagic shock rats can affect the expression of EPCR in human umbilical vein endothelial cells by gene and protein level.