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目的了解市售乳制品中是否存在反式脂肪酸,并测定其存在的种类和含量。方法随机抽取市售的奶粉、纯牛奶和酸奶3种奶制品,采用气相色谱法外标法定量,分别对3种乳制品中反式脂肪酸组成进行测定。结果硬脂酸甲酯(C18∶0)、顺式油酸甲酯(C18∶1-9c)与反式油酸甲酯(C18∶1-9t),顺式亚油酸甲酯(C18∶2-9c,12c)与反式亚油酸甲酯(C18∶2-9t,12t)分离效果良好。3种乳制品中反式脂肪酸均有检出,反式脂肪酸含量为0.129~0.216 g/100 g。结论市售的奶粉和纯牛奶样品检出1种反式脂肪酸,酸奶样品检出2种反式脂肪酸。
Aim To understand the presence or absence of trans fatty acids in commercially available dairy products and determine their presence and content. Methods Three kinds of dairy products, milk powder, pure milk and yoghurt, were randomly selected and quantified by gas chromatography external standard method. The compositions of trans fatty acids in three kinds of dairy products were determined respectively. Results Methyl stearate (C18: 0), methyl cis-oleate (C18: 1-9c) and trans- methyl oleate (C18: 1-9t), methyl cis-linoleate 2-9c, 12c) was separated from trans methyl linoleate (C18: 2-9t, 12t). Trans-fatty acids were detected in the three kinds of dairy products, and trans-fatty acid content was 0.129-0.216 g / 100 g. Conclusion One trans fatty acid was detected on the commercially available milk powder and pure milk samples and two trans fatty acids were detected on the yogurt samples.