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目的 :研究锌、维生素 C、维生素 E、二巯基化合物这几类抗氧化剂对砷染毒小鼠毒作用的拮抗作用。方法 :昆明种小鼠饮用含砷水同时通过灌胃给予不同种类的抗氧化剂 ,8周后 ,测定小鼠肝脏超氧化物岐化酶 (SOD)活性及丙二醛 (MDA)含量。结果 :干预后 ,与砷染毒对照组比较 :维生素 C组、维生素 E组、巯基组的 SOD活性升高 ,MDA含量下降 (P<0 .0 5 )。锌制剂组仅 SOD活性升高 ;与正常对照组比较 :维生素 C组、维生素 E组的 SOD活性和 MDA含量达到正常对照组水平 ,锌制剂组的 SOD活性与巯基组的 MDA含量分别达到正常对照组水平 (P>0 .0 5 )。结论 :一定剂量的锌制剂、维生素 C、维生素 E、二巯基化合物对砷染毒小鼠毒作用有拮抗作用。其中维生素 C、维生素 E的拮抗作用强 ,能完全纠正染砷小鼠的脂质过氧化的损伤 ,而锌制剂与二巯基化合物有一定的拮抗作用。
Objective: To study the antagonism of zinc, vitamin C, vitamin E and dimercapto compounds on the toxic effects of arsenic-exposed mice. Methods: Kunming mice were challenged with arsenic-containing water and given different kinds of anti-oxidants by gavage. After 8 weeks, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the liver were measured. Results: Compared with the control group after arsenic exposure, the activity of SOD and the content of MDA decreased in vitamin C group, vitamin E group and thiol group (P <0.05). Compared with normal control group, SOD activity and MDA content in vitamin C group and vitamin E group reached the level of normal control group, SOD activity in zinc preparation group and MDA content in sulfhydryl group reached normal control Group level (P> 0.05). Conclusion: A certain dose of zinc preparations, vitamin C, vitamin E and dimercapto compounds have antagonistic effect on arsenic-exposed mice. Among them, the antagonism of vitamin C and vitamin E is strong, which can completely correct the lipid peroxidation damage of arsenic-stained mice, while the zinc preparation and the dimercapto compounds have some antagonistic effects.