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目的探索手足口病患儿肠道菌群结构特征,了解肠道菌群与疾病的相关性。方法收集手足口病住院患儿32例和健康儿童14例粪便样本,提取肠道菌群总脱氧核糖核酸(DNA),16SrDNA-V3区聚合酶链反应(PCR)扩增,利用变性梯度凝胶电泳(DGGE)分子指纹图谱和测序技术,分析手足口病患儿肠道菌群组成结构、多样性、相似性等生态学特征。结果手足口病患儿与健康儿童相比,DGGE指纹图谱的条带数和Shannon-Wiener指数均显著降低(t=3.92,P<0.05;t=3.24,P<0.05),肠道菌群多样性降低;比较两组相似性系数和相似性系数累积曲线,手足口病患儿组内相似性降低(Z=-9.12,P<0.05),菌群结构更趋不同;优势条带切胶测序结果表明,手足口病患儿以厚壁菌门、变形菌门、拟杆菌门为主,肠道优势菌属可能发生变化。结论手足口病患儿肠道菌群结构、组成发生变化,疾病的发生与肠道菌群具有一定的相关性。
Objective To explore the structure of intestinal microflora in children with hand-foot-mouth disease and to understand the correlation between intestinal microflora and disease. Methods 32 cases of HFMD in hospitalized children and 14 healthy children were collected for stool samples. Total DNA extracted from intestinal flora and 16S rDNA-V3 region were amplified by polymerase chain reaction (PCR) Electrophoresis (DGGE) molecular fingerprinting and sequencing technology to analyze the intestinal flora composition, diversity, similarity and other ecological characteristics of children with hand-foot-mouth disease. Results The number of bands and Shannon-Wiener index of DGGE fingerprints of HFMD children were significantly lower than those of healthy children (t = 3.92, P <0.05; t = 3.24, P <0.05) (Z = -9.12, P <0.05). The flora structure was more and more different. The dominant bands were cut by gel electrophoresis The results showed that children with HFMD were mainly composed of Firmicutes, Proteobacteria and Bacteroidetes, and the dominant intestinal bacteria might change. Conclusion The structure and composition of intestinal microflora of children with hand-foot-mouth disease have changed, and the occurrence of the disease has some correlation with intestinal microflora.