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已实现了用日本山茶(Camellia japonica)与茶树(C. sinensis)子叶片断再生小植物体。如果没有愈伤组织形成的培养基,子叶表面发生分化,在子叶膨胀部分的表面直接形成了不定胚,从组织学上观察这些不定胚是很明显的。不定胚总是不取决于生长调节剂的浓度与存在度,在子叶片断上按相同的方式形成。在用GA_3(赤霉素)添加的培养基上,日本山茶的不定胚长出幼茎与根,几个月继代培养后,移植了许多小植物体,而且随着继代培养,体细胞胚在胚轴上形成了另外的胚,在GA_3培养基上,在胚的继续产生的始终,这个现象是永存的。在用10.0毫克/升BA(N6-苄基腺嘌呤)与0.5毫克/升IBA(吲哚—3—丁酸)添加的培养基上,茶树的不定胚长出幼茎与根;但在用GA_3添加的培养基上则不能。考虑通过子叶片断迅速大量的无性系繁殖可从山茶属选择杂交的1粒种子获得。
Regeneration of plantlets with Camellia japonica and C. sinensis cotyledons has been achieved. Without callus formation medium, the cotyledon surface is differentiated and adventitious embryos are formed directly on the surface of the cotyledon bulging section, and it is obvious from the histological observation of these adventitious embryos. Adventitious embryos are always formed in the same manner on the cotyledon segments regardless of the concentration and abundance of growth regulators. In GA_3 (Gibberellin) added medium, Japanese camellia adventitious embryos grow young stems and roots, a few months after subculture, transplanting a number of plantlets, and with subculture, somatic cells The embryo forms additional embryos on the hypocotyls. This phenomenon is perpetuated on the GA3 medium during the continuous production of embryos. The adventitious embryos of tea plants grew young stems and roots on medium supplemented with 10.0 mg / L BA (N6-benzyladenine) and 0.5 mg / L IBA (indole-3-butyric acid) GA_3 added medium can not. Consider rapid bulk clonal propagation through cotyledon segments Obtainable from 1 seed selected from Camellia hybrids.