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为了解发热伴血小板减少综合征布尼亚病毒(SFTSV)的传播机制,采集了山东疫区家养牛、羊和狗等动物体表蜱,分类鉴定后,通过Real-time PCR筛查、病毒分离培养和基因组序列分析等方法分离鉴定蜱中的病毒。所采集的蜱,以长角血蜱为主,占91.4%。其中3头SFTSV核酸检测阳性,阳性率为2.14%,并在其中一份羊体表蜱标本中分离到SFTSV病毒,命名为SDLZTick12。序列分析显示与我国在不同省份患者标本中分离的病毒全基因序列具有高度同源性,且病毒的抗原性和生长特性与人源病毒相同。本研究首次在山东疫区蜱中分离到新型布尼亚病毒,并与人源病毒进行了系统比较研究,提示蜱可能为该新病原体的传播媒介,对疾病的防控具有重要的指导意义。
In order to understand the transmission mechanism of fever with thrombocytopenic syndrome Bunyavirus (SFTSV), the body ticks of domestic animals such as cattle, goats and dogs were collected from Shandong Province. After classification and identification, they were screened by Real-time PCR. Isolation and culture and genomic sequence analysis methods to isolate and identify ticks in the virus. The collected ticks, mainly to the long-horned ticks, accounting for 91.4%. Three SFTSV nucleic acids were detected positive, with a positive rate of 2.14%. SFTSV virus was isolated from one of the sheep tick tick specimens and named as SDLZTick12. Sequence analysis showed highly homologous to the whole genome sequence of the virus isolated from the patient samples in different provinces in China, and the antigenicity and growth characteristics of the virus were the same as those of the human virus. In this study, a new Bunyavirus was isolated from ticks in Shandong province for the first time and compared with human viruses. It is suggested that tick may be the transmission medium of this new pathogen, which is of great guiding significance for disease prevention and control.