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克隆并测定了引起产科新生儿腹泻暴发的Ｐ２［６］ 、Ｇ４ 型轮状病毒（ＢＮ 株）ＶＰ４ 的ＶＰ８片段和ＶＰ７ 编码基因的核苷酸序列，并据此推导出其氨基酸序列。与相应标准株和地方株（ 包括有毒株和无毒株） 比较的结果表明，所测ＶＰ８ 序列与相同型别（Ｐ２［６］） 的标准株Ｍ３７（ 无毒株） 和ＳＴ３（ 无毒株） 、地方株Ｎ１６（ 无毒株） 和ＶＥ７１５６（ 有毒株） 之间的同源性为９２－８ ％ ～９８－６ ％ ，胰酶作用位点各毒株间相同；位于ａａ４９ 、ａａ５０ 、ａａ５２ 、ａａ５３ 、ａａ７８ 处的氨基酸在有毒株与无毒株间（ 包括ＢＮ株） 不同，但分别保守。ＶＰ７ 基因与同型（Ｇ４） 标准株ＳＴ３（Ａ 亚型／ 无毒株） 和ＶＡ７０（Ｂ 亚型／ 有毒株） 、意大利地方株ＰＶ５２４９（Ａ 亚型／ 有毒株）和北京地方株ＣＲ１１７（ 有毒株） 、同型猪有毒株Ｇｏｔｔ 之间的同源性为９１－４ ％ ～９７－８ ％ ，其中与Ａ亚型的同源性为９５－５ ％ ～９６－３ ％ ，而与Ｂ亚型的同源性为９１－４ ％ ，提示ＶＰ７ 为Ｇ４Ａ 亚型，位于ａａ３８ 、ａａ７８ 、ａａ１４５ 、ａａ２３８ 位点的氨基酸在有毒株与无毒株之间不同，但分别保守。分析了虽为Ｐ２［６］ 型却反常地引起新生儿?
The nucleotide sequence of VP8 and VP7 coding genes of VP4, P2 of genotype G4 rotavirus (BN strain), which caused obstetric neonatal diarrhea, was cloned and sequenced. Based on this, the deduced amino acid sequence was obtained. The results of comparison with the corresponding standard strains and local strains (including virulent strains and non-virulent strains) showed that the measured VP8 sequences were identical to the standard strains M37 (avirulent strain) and ST3 (avirulent strain) of the same type (P2 [6] ), The homology between local strains N16 (non-virulent strains) and VE7156 (virulent strains) was 92-8% ~ 98-6%, and the same among the isolates of pancreatic loci was located; aa49, aa50, aa52 The amino acids at aa53 and aa78 differed between the virulent and non-virulent strains (including the BN strain), but conserved separately. The VP7 gene was compared with the isolates of the same type (G4) ST3 (A subtype / avirulent strain) and VA70 (subtype B / avirulent strain), the Italian local strain PV5249 (subtype A / virulent strain) and Beijing local strain CR117 ). The homology between the homologous porcine virulent strain Gott was 91-4% ~ 97-8%. The homology with the A subtype was 95-5% ~ 96-3% The homology was 91-4%, suggesting that VP7 was a G4A subtype. The amino acids located at aa38, aa78, aa145 and aa238 were different between the virulent and non-virulent strains but conserved respectively. Analysis of the P2 [6] type has caused abnormal newborns?