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目的:优化反胶束法分离和提取溶菌酶的条件,为溶菌酶的工业提取提供参考。方法:以溶菌酶质量浓度为评价指标,通过单因素试验和正交试验考察反胶束团中溴化十六烷基三甲铵(CTAB)浓度、正辛烷与正己醇的配比、氯化钾溶液浓度、被萃取液与萃取液比例对溶菌酶提取工艺的影响。结果:溶菌酶的最优提取条件为CTAB浓度30 mmol·L-1,正辛烷-正己醇(4∶1),KCl浓度0.15 mol·L-1,被萃取液-萃取液(1∶2)。在该条件下,溶菌酶质量浓度1.330 g·L-1。结论:反胶束溶液提取溶菌酶的方法具有液液萃取的优点,溶菌酶不容易变性失活且溶解度较高,值得进一步推广与开发。
OBJECTIVE: To optimize conditions for the separation and extraction of lysozyme by reverse micelles and to provide a reference for the industrial extraction of lysozyme. Methods: The concentration of lysozyme was used as the evaluation index. The concentration of cetyltrimethylammonium bromide (CTAB), the ratio of n-octane to n-hexanol, Effect of potassium concentration, ratio of extract to extract on lysozyme extraction. Results: The optimal extraction conditions for lysozyme were as follows: CTAB concentration 30 mmol·L-1, n-octane-n-hexanol 4:1, KCl 0.15 mol·L- ). Under this condition, the concentration of lysozyme was 1.330 g · L-1. CONCLUSION: The method of reverse micelle extraction of lysozyme has the advantage of liquid-liquid extraction. Lysozyme is not easily denatured and inactivated and has high solubility, so it is worth further promotion and development.