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目的优化大孔树脂纯化芒萁总皂苷的工艺参数。方法分别用AB-8、D-101、Hz-803、HZ-816大孔树脂对芒萁皂苷进行静态吸附与解吸试验,筛选出分离效果最好的一种树脂,并研究该树脂分离芒萁总皂苷的动力学曲线和动态吸附性能。以BHT为对照,考察纯化后的芒萁总皂苷清除DPPH自由基的效果。结果优化的D-101大孔树脂对芒萁皂苷的吸附条件为:流速2BV·h-1时,料液的pH值和浓度分别为5和20.5 mg·ml-1;优化的洗脱条件:流速为2BV·h-1,洗脱溶剂为3BV 70%的乙醇水溶液。纯化后总皂苷含量为64.9%,纯化倍数为2.2倍;纯化后的芒萁总皂苷对DPPH自由基的清除效果比BHT好,浓度为1.0 mg/ml时,芒苷皂苷对DPPH自由基的清除率达95.6%。结论 D-101大孔树脂对芒其皂苷有很好的纯化效果,芒萁皂苷对DPPH自由基有很好的清除作用,是一种很好的抗氧化剂。
Objective To optimize the process parameters of purifying total saponins from macroporous resin. Methods The macroporous resins AB-8, D-101, Hz-803 and HZ-816 were respectively used for the static adsorption and desorption experiments of mangosteen saponins. The best separation resin was screened and the separation of mangosteen Kinetic curves and dynamic adsorption properties of total saponins. Using BHT as a control, the effect of purifying total saponins of Radix Paeoniae Alba was scavenged by DPPH free radical. Results The optimum conditions for the adsorption of mulgules saponins by D-101 macroporous resin were as follows: the flow rate of 2BV · h-1, the pH and concentration of feed solution were 5 and 20.5 mg · ml-1, respectively; The flow rate was 2BV · h-1, and the elution solvent was 3BV 70% aqueous solution of ethanol. After purification, the content of total saponin was 64.9%, and the purification fold was 2.2 times. Purified total saponin from Radix mongholii had stronger DPPH radical scavenging effect than BHT. At the concentration of 1.0 mg / ml, Rate of 95.6%. Conclusion D-101 macroporous resin has a good purification effect on mangosteen saponins. Mangosteen saponin has a good scavenging effect on DPPH free radicals and is a good antioxidant.