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目的:分析柑橘、油菜和紫云英蜜中的特征黄酮类组分。方法:采用UPLC-MS/MS同时分析油菜蜜、紫云英蜜和柑橘蜜及其蜜源花中的黄酮类组分。色谱柱采用Inertsil ODS-3柱(2.1 mm×75 mm,2μm),柱温40℃,以甲醇-0.02%甲酸为流动相梯度洗脱,流速为0.4 m L·min~(-1);以儿茶酸、表儿茶酸、芦丁、芸香柚皮苷、桑黄素、杨梅素、山柰酚、生松素等16个黄酮类对照品作为对照,采用电喷雾离子源(ESI),多反应监测(MRM)进行分析。结果:不同蜜源蜂蜜中的特征黄酮类组分及其含量(从高到低排列)有所不同:柑橘蜜的特征组分有柚皮素、木犀草素、芹菜素和汉黄芩素;油菜花蜜的特征组分有柚皮素、槲皮素和汉黄芩素;紫云英蜜的特征组分有槲皮素、山柰酚、芹菜素和汉黄芩素。结论:该方法灵敏度高,以蜜源花作为“对照”寻找蜂蜜中的特征成分具有数据可靠、可溯源性,并对蜜源花中化学成分的分析具有指导意义。
Objective: To analyze the characteristic flavonoids in citrus, rape and millet honey. Methods: The flavonoids in rapeseed, milk vetch, citrus honey and nectarine were analyzed by UPLC-MS / MS simultaneously. The column was eluted with Inertsil ODS-3 column (2.1 mm × 75 mm, 2 μm) at a column temperature of 40 ° C with a gradient of methanol to 0.02% formic acid as mobile phase at a flow rate of 0.4 m L · min -1. Sixteen flavonoid reference materials, including catechin, epicatechin, rutin, rutin, naringin, myricetin, myricetin, kaempferol and sophorid, were used as control. Electrospray ionization (ESI) Multiple Reaction Monitoring (MRM) was analyzed. Results: The characteristic flavonoids and their contents (arranged from highest to lowest) in different nectar honey were different: the characteristic components of citrus honey were naringenin, luteolin, apigenin and wogonin; rape nectar The characteristic components of naringenin, quercetin and wogonin; the characteristic components of milk vetch honey are quercetin, kaempferol, apigenin and wogonin. Conclusion: The method has high sensitivity. It is reliable and traceable to find the characteristic components of honey by using “honey” flower as the “control”, and is of guiding significance for the analysis of chemical constituents in honey.