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目的:探讨肝癌介入治疗前后多药耐药相关蛋白(multidrug resistance-associated protein,MRP)基因的表达情况及机制。方法:28例无手术切除指征或拒绝手术治疗的肝癌患者行肝动脉化疗栓塞术治疗,对治疗前后肝癌组织部位行细针穿刺活检,利用实时荧光定量PCR法检测MRP-1、MRP-2、MRP-3、MRP-5基因表达水平。结果:肝动脉化疗栓塞术治疗后MRP-1、MRP-3、MRP-5基因表达水平较治疗前明显增高(P<0.05),治疗前后MRP2基因表达水平差异无统计学意义(P>0.05)。结论:肝癌介入治疗后产生的获得性多药耐药可能与MRP-1、MRP-3、MRP-5基因表达增高有关。
Objective: To investigate the expression and mechanism of multidrug resistance-associated protein (MRP) gene in human hepatocellular carcinoma before and after interventional therapy. Methods: Twenty-eight patients with hepatocellular carcinoma without surgical indication or surgical treatment were treated with transcatheter arterial chemoembolization. Fine-needle aspiration biopsy was performed on the liver cancer tissue before and after treatment. MRP-1 and MRP-2 were detected by real-time fluorescence quantitative PCR , MRP-3, MRP-5 gene expression levels. Results: The mRNA expression of MRP-1, MRP-3 and MRP-5 after hepatic artery chemoembolization was significantly higher than that before treatment (P <0.05), but there was no significant difference in MRP2 gene expression between before and after treatment (P> 0.05) . Conclusion: The acquired multidrug resistance induced by interventional therapy of liver cancer may be related to the increase of MRP-1, MRP-3 and MRP-5 gene expression.