论文部分内容阅读
目的对参附注射液中的人参皂苷和生物碱成分进行定性,并且对参附注射液中主要成分在大鼠体内的药物动力学过程进行研究。方法运用LC-MS对参附注射液中的成分进行定性,并测定大鼠血清中多种人参皂苷的浓度,计算其药动学参数。结果参附注射液中定性出16种人参皂苷成分和10种生物碱成分。人参皂苷Rd、Rg1、Rb1、Ro、Rc、Rb2的T12α分别为0.32±0.25、0.11±0.04、0.30±0.24、0.11±0.02、0.24±0.19、0.23±0.12 h,人参皂苷Rd、Rb1、Rc、Rb2的T12β分别10.25±0.39、21.31±3.64、13.95±2.56、15.06±1.54 h。结论该方法简便、灵敏、特异,适用于血清中6种人参皂苷成分的药物动力学研究;人参皂苷Rg1、Ro在大鼠体内的分布消除速度较快,Rd、Rb1、Rc、Rb2分布消除较慢。参附注射液体外、体内成分的分析为组分配伍研究建立了可靠的方法。
Objective To characterize the components of ginsenosides and alkaloids in Shenfu injection, and to study the pharmacokinetics of the main components in Shenfu injection in rats. Methods The composition of Shenfu injection was characterized by LC-MS. The concentrations of various ginsenosides in serum of rats were determined and their pharmacokinetic parameters were calculated. Results Shenfu injection qualitative identification of 16 kinds of ginsenosides and 10 kinds of alkaloid ingredients. T12α of ginsenoside Rd, Rg1, Rb1, Ro, Rc and Rb2 were 0.32 ± 0.25, 0.11 ± 0.04, 0.30 ± 0.24, 0.11 ± 0.02, 0.24 ± 0.19 and 0.23 ± 0.12 h, respectively, and ginsenoside Rd, Rb1, Rc, T12β of Rb2 were 10.25 ± 0.39, 21.31 ± 3.64, 13.95 ± 2.56 and 15.06 ± 1.54 h, respectively. Conclusions The method is simple, sensitive and specific and suitable for the pharmacokinetic study of six kinds of ginsenosides in serum. The distribution of ginsenosides Rg1 and Ro in rats is faster and the distribution of Rd, Rb1, Rc and Rb2 is less SLOW. Shen Fu injection of liquid, body composition analysis for the component compatibility study established a reliable method.