论文部分内容阅读
目的:探讨二巯丙磺钠(DMPS)对四次甲基二砜四胺(TETS)拮抗GABA受体的影响。方法:通过急性毒性试验观察DMPS和TETS对小鼠的影响;利用氨基酸自动分析仪测定小鼠脑内游离氨基酸的变化;通过宏观放射自显影术观察不同实验组中[~3H]-GABA与其受体结合情况。结果:与NS对照组比较,icv和ip DMPS后小鼠的惊厥数从20只下降至4只和2只;小鼠脑内游离GABA含量与DMPS保护组和生理盐水组比较,DMPS对照组和TETS对照组含量均有变化[μmol/g:(2.09±0.05)和(2.67±0.15),vs(2.40±0.10)和(2.41±0.21)];在NS对照组、DMPS对照组、TETS对照组和DMPS保护组中,小鼠脑内游离的谷氨酸(Glu)含量分别是(12.3±1.2),(12.0±0.8),(10.2±0.6)和(11.8±1.0)μmol/g。与缓冲液对照组大鼠脑片中不同脑区[(0.102±0.003),(0.109±0.005),(0.128±0.007)和(0.125±0.008)]比较,TETS组放射自显影图像中大脑皮质、海马、间脑、脑干的光密度显著降低[(0.084±0.008),(0.081±0.009),(0.094±0.006),和(0.081±0.006)];DMPS+TETS组中不同脑区的光密度[(0.116±0.008),(0.125±0.011),(0.129±0.005)和(0.128±0.010)]维持或高于正常水平[(0.102±0.003),(0.109±0.005),(0.128±0.007)和(0.125±0.008)]。结论:DMPS通过增加脑内GABA与受体的结?
Objective: To investigate the effects of sodium dimercaptopropane sulfonate (DMPS) on GABA receptor antagonist by tetrakis methyldisulfone tetramine (TETS). Methods: Acute toxicity test was used to observe the effects of DMPS and TETS on mice. Amino acid autoanalyzer was used to detect the changes of free amino acids in mice brain. The effects of [~ 3H] -GABA and its receptor in different experimental groups were observed by macroscopic autoradiography Body with the situation. Results: Compared with the NS control group, the number of convulsions in mice after icv and ip DMPS decreased from 20 to 4 and 2. Compared with the DMPS-protected group and the saline group, the free GABA content in the brain of the DMPS control group and The levels of TETS in the control group were all changed [μmol / g (2.09 ± 0.05) and (2.67 ± 0.15) vs (2.40 ± 0.10) and (2.41 ± 0.21) (12.3 ± 1.2), (12.0 ± 0.8), (10.2 ± 0.6) and (11.8 ± 1.0) μmol / g, respectively, in the mouse brain and DMPS-protected group. In the TETS group, in the autoradiogram, the cerebral cortex, the cerebral cortex, and the cortical area of the TETS group were significantly decreased compared with those in the brain regions of the control group ([(0.102 ± 0.003), (0.109 ± 0.005), (0.128 ± 0.007) and (0.125 ± 0.008) The optical densities of hippocampus, diencephalon and brain stem decreased significantly in the DMPS + TETS group [(0.084 ± 0.008), (0.081 ± 0.009), (0.094 ± 0.006), and (0.081 ± 0.006) [(0.116 ± 0.008), (0.125 ± 0.011), (0.129 ± 0.005) and (0.128 ± 0.010)] were maintained at or above the normal levels [(0.102 ± 0.003), (0.109 ± 0.005), (0.125 ± 0.008)]. Conclusion: DMPS increases the number of GABA receptors in the brain.