比较性基因组杂交(CGH)可用以检查肿瘤DNA的过表达及低表达。进行CGH定量分析的前提是必须将分子细胞遗传学、荧光显微术及数字化图像处理等3种方法紧密结合起来。这一分析程序的基础是载有正常中期染色体的载玻片,其中含有已经原位杂交标记了的待检和对照的DNA。 对所测标本必须完成如下步骤:荧光图像拍摄、荧光图像的光学调整、强度标准化以计算RATIO图像、制成核型图、CGH总核型图计算、结果评估。 Comparative genomic hybridization (CGH) can be used to examine overexpression and low expression of tumor DNA. The premise of CGH quantitative analysis is that the three methods of molecular cytogenetics, fluorescence microscopy, and digital image processing must be closely combined. The basis of this analysis procedure is a slide containing a normal metaphase chromosome containing the DNA to be examined and the control that has been labeled in situ. The following steps must be completed for the specimens tested: fluorescence image capture, optical adjustment of fluorescence images, and intensity normalization to calculate RATIO images, make karyotype maps, CGH total karyotype calculations, and evaluation of results.