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目的 研究中药“参芪脑保”对脑缺血 再灌注损伤的治疗作用。方法 用线栓法制作大鼠大脑中动脉缺血 6小时再灌注模型 ,将大鼠随机分为对照组 (2 0只 )和参芪脑保治疗组 (2 0只 )。治疗组于术后 3小时始连续 7天给予参芪脑保灌胃治疗 ,观察神经功能缺损评分变化。各组大鼠于用药 7天后处死 ,行TTC和HE染色 ,测定脑梗死体积及神经元损伤情况。结果 参芪脑保治疗组大鼠 4 8小时死亡数 5只 (占2 5 % )低于对照组 10只 (占 5 0 % ) ,而 7天存活数 15只 (占 75 % )高于对照组 10只 (占 5 0 % )。参芪脑保治疗组梗死灶体积 (2 6 .5± 5 .2 )mm3 明显小于对照组 (4 1.9± 7.3)mm3 (P <0 .0 0 1) ,神经元缺失、脑梗死灶内出血较对照组减小。结论 “参芪脑保”对脑缺血 再灌注损伤有保护作用。
Objective To study the therapeutic effect of Shenqi Naobao on cerebral ischemia-reperfusion injury. Methods Rat model of middle cerebral artery occlusion (MCAO) was established by reperfusion. The rats were randomly divided into control group (20 rats) and Shenqi Naobao protective group (20 rats). The treatment group was given Shenqi Naobao gavage for 3 days after operation for 7 consecutive days to observe the change of neurological deficit score. Rats in each group were sacrificed 7 days after treatment, TTC and HE staining were performed to determine the infarct volume and neuronal damage. Results In Shenqi Naobao treatment group, 5 deaths occurred in 48 hours (25%) and 10 cases (50%) died in control group, while 15 days (75%) survived in 7 days was higher than that in control group Group 10 (50%). The volume of infarction in Shenqi Naobao treatment group was (26.5 ± 5.2) mm3, which was significantly lower than that in control group (4.19 ± 7.3) mm3 (P <0.01), neuron loss and cerebral infarction The control group decreased. Conclusion Shenqi Naobao has a protective effect on cerebral ischemia-reperfusion injury.