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目的 了解临床分离株红霉素耐药粪肠球菌耐药特点及耐药基因erm(A)、erm(B)和erm(C)分布特点及耐药机制. 方法 采用BD Phoenix-100全自动细菌鉴定/药敏系统对2010～2016年临床分离的313株粪肠球菌进行药敏试验,并用微量肉汤稀释法检测红霉素的MIC值,比较红霉素耐药组和红霉素敏感组细菌的耐药性差异,使用PCR方法检测erm(A)、erm(B)和erm(C)基因并分析其分布特点. 结果 313株粪肠球菌主要分离自患者的中段尿、血液、胆汁和分泌物,红霉素耐药组和红霉素敏感组细菌对万古霉素、利奈唑胺、氨苄西林、替考拉宁和呋喃妥因的敏感性高,对庆大霉素的耐药率均＞90％.红霉素耐药基因erm(A)、erm(B)和erm(C)基因检出比例分别为3.51％、66.77％和 0.32％;携带红霉素耐药基因erm(A)细菌利奈唑胺(LZD)耐药为81.82％.粪肠球菌对红霉素耐药率为76.04％.结论 红霉素耐药株粪肠球菌主要携带erm(B)基因.红霉素耐药基因erm(A)阳性菌株LZD的MIC值偏高.“,”Objectives To determine the drug resistance of E.faecalis isolates from patients and to investigate the distribution of the erythromycin resistance genes erm(A),erm(B),and erm(C). Methods The BD Phoenix-100automated bacterial identification system was used to perform a clinical susceptibility test on 313strains of E.faecalisisolated from 2010to 2016.The micro-broth dilution method was used to detect the MIC of erythromycin with respect to E.faecalis. PCR was used to detect the erythromycin resistance genes erm(A),erm(B),and erm(C). Results E.faecalis was mainly found in mid-stream urine,blood,bile,and secretions.Three hundred and thirteen strains of E.faecalis were highly sensitive to linezolid,ampicillin,teicoplanin,and nitrofurantoin but were resistant to gentamicin at a rate of 90％or higher.The erm(A)gene was detected at a rate of 3.51％,the erm(B)gene was detected at a rate of 66.77％, and the erm(C)gene was detected at a rate of 0.32％.The erm(B)gene was detected in 81.82％of the strains of linezolid- resistant E.faecalis.E.faecalis was resistant to erythromycin at a rate of 76.04％,and resistant strains mainly carried the erm (B)gene. Conclusion Linezolid and vancomycin can be used clinically to treat an E.faecalis infection, but the drug resistance of E.faecalis should be monitored.