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食源性致病金黄色葡萄球菌的筛查和检测备受关注,传统的生化鉴定方法存在耗时长和灵敏度低等缺陷,本文以原核生物核糖体小亚基为主要研究对象,利用16S核糖体RNA测序和核糖体基因分型技术对46株金黄色葡萄球菌进行了鉴定和分型,结果表明:API-STAPH生化方法鉴定的准确率为93%,而分子生物学鉴定的准确率≥99%;全自动微生物核糖体基因分型系统将46株金黄色葡萄球菌分为22个Ribogroup。上述研究将食源性金黄色葡萄球菌的鉴定上升到“亚型”的高度,16S核糖体RNA测序和核糖体分型技术可为食源性致病菌的风险监测提供有力的技术支持。
The screening and testing of foodborne pathogenic Staphylococcus aureus have drawn much attention. The traditional biochemical identification methods have the defects of time-consuming and low sensitivity. In this paper, the small subunit of prokaryotic ribosomes as the main research object, the use of 16S ribosome RNA sequencing and ribosomal genotyping 46 strains of Staphylococcus aureus were identified and typed, the results showed that: API-STAPH biochemical method to identify the accuracy of 93%, while the molecular biology of the accuracy of identification ≥ 99% ; 46 strains of Staphylococcus aureus were divided into 22 Ribogroups by a fully automated microbial ribosomal genotyping system. The above research raised the identification of foodborne Staphylococcus aureus to the level of “subtype”. The 16S ribosomal RNA sequencing and ribosome typing technology can provide strong technical support for the risk monitoring of foodborne pathogens .