论文部分内容阅读
目的通过研究KAI1基因在人类喉鳞状细胞癌及转移淋巴结中的蛋白表达情况,试图阐明KAI1基因蛋白的表达与喉癌转移之间的关系。方法取喉鳞状细胞癌组织标本蜡块40例及转移的淋巴结13例,以癌旁正常组织标本蜡块30例作为对照,采用免疫组织化学SP法,检测KAI1单克隆抗体在喉鳞状细胞癌、转移淋巴结及癌旁正常组织标本中蛋白的表达,并结合患者临床病理资料进行统计学分析。结果KAI1基因在癌旁正常组织、喉鳞状细胞癌及转移淋巴结中的表达率分别为93.33%(28/30)、62.50%(25/40)、30.77%(4/13),且各组之间差异均有统计学意义(P<0.05)。KAI1基因的蛋白表达与喉鳞状细胞癌的临床分期差异有统计学意义(P<0.05),与性别、年龄、原发部位、病理学分级及有无淋巴结转移无关(P>0.05)。结论KAI1基因在喉鳞状细胞癌转移淋巴结中的表达较在喉鳞状细胞癌中的表达呈显著性下降,该基因可能在喉癌转移过程中起一定作用。
Objective To study the expression of KAI1 in human laryngeal squamous cell carcinoma and metastatic lymph nodes and to elucidate the relationship between KAI1 gene expression and laryngeal carcinoma metastasis. Methods 40 cases of laryngeal squamous cell carcinoma tissue samples and 13 cases of lymph node metastasis were taken as control. The immunohistochemical SP method was used to detect the expression of KAI1 monoclonal antibody in laryngeal squamous cells Cancer, lymph node metastasis and adjacent normal tissue samples of protein expression, combined with clinical and pathological data of patients for statistical analysis. Results The expression rates of KAI1 in normal tissues, laryngeal squamous cell carcinomas and metastatic lymph nodes were 93.33% (28/30), 62.50% (25/40) and 30.77% (4/13) respectively, The differences were statistically significant (P <0.05). The expression of KAI1 protein was significantly different from that of laryngeal squamous cell carcinoma (P <0.05), but not related to sex, age, primary site, pathological grade and lymph node metastasis (P> 0.05). Conclusion The expression of KAI1 in metastatic lymph nodes of LSCC is significantly lower than that in LSCC, which may play a role in the metastasis of laryngeal squamous cell carcinoma.