目的:提高脾肾两助丸的质量标准,建立脾肾两助丸中芍药苷的含量测定方法。方法:采用高效液相色谱法,以乙腈-0.05 mol.L-1磷酸二氢钾(10∶90)为流动相,色谱柱为Kromasil-C18(4.6 mm×250 mm,5μm),检测波长230 nm。结果:芍药苷进样量在0.106 2～1.062μg与峰面积线性关系良好(r=0.999 95),平均回收率为96.83%。结论:方法专属性强,准确、简便,可用于脾肾两助丸的质量控制。 Objective: To improve the quality standard of spleen and kidney Liangzhu pill and to establish the method of determination of paeoniflorin in spleen and kidney Liangzhu pill. Methods: The mobile phase consisted of Kromasil-C18 (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.05 mol·L-1 potassium phosphate monobasic (10:90) at a detection wavelength of 230 nm. Results: The calibration curve of paeoniflorin showed a good linear relationship (r = 0.999 95) with the peak area of ​​0.106 2 ~ 1.062 μg and the average recovery was 96.83%. Conclusion: The method is specific, accurate and simple, and can be used for quality control of spleen and kidney two pills.