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应用淋巴结改良直接法、短期培养法及骨髓短期培养法对２４例小儿恶性淋巴瘤进行细胞遗传学研究，２３例获得满意分裂相。结果表明：全部淋巴结染色体及４例骨髓染色体显示克隆性异常。非何杰金淋巴瘤染色体众数多为二倍体及亚二倍体，分布似与组织学分型有关；何杰金淋巴瘤染色体众数从亚二倍体到四倍体。最常见的染色体结构异常为６ｑ－。本文出现的染色体相互易位大都涉及到１４ｑ３２及其附近，证实１４ｑ３２的断裂重排与恶性淋巴瘤的发生有关。此外，本文还提示分别定位于１ｐ１３、６ｑ２２－２４及１７ｐ上的癌基因ＮＲＡＳ，ｃ－ｍｙｂ，及肿瘤抑制基因Ｐ５３在恶性淋巴瘤的发生发展中也可能起着一定作用。
Twenty-four cases of pediatric malignant lymphoma were studied by cytogenetics using the method of direct lymph node improvement, short-term culture and bone marrow short-term culture, and 23 cases were divided into two groups. The results showed that: All lymph node chromosomes and 4 cases of bone marrow chromosomes showed clonal abnormalities. Non-Hodgkin’s lymphoma chromosomes are mostly diploid and sub-diploid, the distribution seems to be related to histological type; Hodgkin lymphoma chromosomes from sub-diploid to tetraploid. The most common chromosomal structural abnormalities are 6q-. The chromosomal reciprocal translocations in this paper are mostly related to 14q32 and its vicinity, confirming the 14q32 rupture rearrangement associated with the occurrence of malignant lymphoma. In addition, we also suggest that oncogenes NRAS, c-myb, and tumor suppressor gene P53 located on 1p13, 6q22-24 and 17p, respectively, may also play a role in the development of malignant lymphoma.