应用Ｈｕ或５Ｆｕ和鼠干细胞因子（ｍＳＣＦ）及鼠白细胞介素３（ｍＩＬ３）培养含金属硫蛋白（ＭＴ）启动子和增强子序列、ｂｃｒａｂｌｃＤＮＡ（ｐ２１０）序列及ＳＶ４０剪切和Ｐｏｌｙ（Ａ）信号序列的转基因慢粒白血病小鼠骨髓细胞，观察化疗药物及细胞因子单独或联合应用对ＭＴ／ｐ２１０（ｂｃｒａｂｌ）转基因小鼠骨髓细胞生长的影响，并采用ＲＴＰＣＲ分析培养前后骨髓细胞中转基因的表达。结果表明，ｍＳＣＦ和ｍＩＬ３联合应用时，明显促进骨髓细胞增殖及集落形成；不加细胞因子培养，则细胞增殖和转基因表达均受抑制；Ｈｕ或５Ｆｕ与ｍＳＣＦ及ｍＩＬ３联合应用时，同样抑制细胞增殖及转基因表达。提示联合应用化疗药物及细胞因子治疗ＣＭＬ是具有极好应用前景的策略。 Using Hu or 5Fu and mouse stem cell factor (mSCF) and mouse interleukin3 (mIL3) to culture metallothionein (MT) promoter and enhancer sequences, bcrablcDNA (p210) sequence and SV40 scissor The effect of chemotherapeutic drugs and cytokines alone or in combination on the growth of bone marrow cells in MT/p210 (bcrabl) transgenic mice was observed by cutting the bone marrow cells of the transgenic chronic myeloid leukemia mice with Poly(A) signal sequence. PCR analysis of transgene expression in bone marrow cells before and after culture. The results showed that the combination of mSCF and mIL3 could significantly promote the proliferation of bone marrow cells and the formation of colony; if no cytokine was added, the proliferation and transgene expression were inhibited; Hu or 5Fu was combined with mSCF and mIL3. At the same time, it also inhibits cell proliferation and transgene expression. It is suggested that the combination of chemotherapeutic drugs and cytokines in the treatment of CML is a strategy with excellent application prospects.