CFL 1 RNAi慢病毒的构建及其对人脐静脉内皮细胞的增殖抑制作用

来源 :卒中与神经疾病 | 被引量 : 0次 | 上传用户:winnerlb
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目的 观察干扰CFL1 对 HUVEC的增殖抑制作用.方法 以 cfl1 基因为模板,设计 RNA干扰靶点,构建 cfl1 基因 RNAi 慢病毒;慢病毒感染人脐静脉内皮细胞 HUVEC 后采用 realtimePCR 鉴定CFL1RNAi慢病毒感染效果,采用CCK-8 法检测 HUVEC细胞的增殖抑制情况.结果 CFL1 RNAi慢病毒干扰 HUVEC细胞后 realtimePCR证实CFL1 RNAi慢病毒可下调 cfl1 基因的表达;CCK-8 法证实 cfl1 基因表达下调可抑制 HUVEC 细胞的增殖.结论 CFL1 RNAi 慢病毒感染 HUVEC 细胞后可明显抑制 cfl1 基因表达,抑制 HUVEC细胞增殖.
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