以类离子液体碳糊电极(CILE)为基体电极,采用滴涂法和利用静电吸附作用,制备了Hb/Fe3O4/CILE修饰电极,研究了Hb的直接电化学及其电催化行为,建立了H2O2的计时安培测定新方法。结果表明:Hb在该修饰电极上,Hb呈现了一对准可逆的氧化还原峰,且其在该修饰电极表面表观覆盖度为2.65×10-9moL/cm2;电子转移速率常数为1.35/s;表观米氏常数为1.59×10-5mol/L。在1.0×10-6~4.0×10-5mol/L范围内,催化电流与H2O2浓度呈线性关系(r=0.9976),检出限为3.0×10-7mol/L(S/N=3)。 The Hb / Fe3O4 / CILE modified electrode was prepared by a drop-coating method and electrostatic adsorption using CILE as the substrate electrode. The direct electrochemical and electrocatalytic behaviors of Hb were studied, and H 2 O 2 A new method of chronoamperometry. The results show that Hb exhibits a quasi-reversible redox peak at the modified electrode, and its apparent coverage on the surface of the modified electrode is 2.65 × 10-9moL / cm2. The electron transfer rate constant is 1.35 / s ; Apparent Michaelis constant is 1.59 × 10-5mol / L. Under the concentration range of 1.0 × 10-6 ~ 4.0 × 10-5mol / L, the linear relationship between the catalytic current and H2O2 concentration was obtained (r = 0.9976). The detection limit was 3.0 × 10-7mol / L (S / N = 3).