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目的 :了解肾上腺糖皮质激素 (激素 )对大鼠阿霉素肾病肾小球损害有无加重作用。方法 :40只雄性SD大鼠 ,随机分成正常对照组 (A组 )、阿霉素组 (B组 )、阿霉毒与激素组 (C组 )及激素组 (D组 )。 B组与 C组一次性经尾静脉注射阿霉素 7mg/ kg。各组于实验的第 16周及 2 8周分别取 5只大鼠的肾脏与血液 ,观察肾脏病理改变 ,测定尿蛋白、血清肌酐、甘油三酯、总胆固醇等指标的变化。结果 :注射阿霉素 1周后尿蛋白开始升高 ,4周后达到 16 8.45±76 .2 8mg/ d。使用激素 12～ 2 4周 (注射阿霉素 16～ 2 8周 )后 :B组尿蛋白、血肌酐、甘油三酯及胆固醇都显著高于 A组 ,同组第 2 8周与第 16周比较 ,尿蛋白、血肌酐升高差异有显著性 ,血浆清蛋白降低有显著差异。C组与 B组比较 ,第16周及第 2 8周尿蛋白、血甘油三酯及胆固醇显著升高 ,第 16周的血肌酐明显升高。 D组尿蛋白、甘油三酯及胆固醇显著高于 A组。B组肾脏病理改变的积分显著高于 A组 ,且第 2 8周显著高于第 16周 ;C组第 16周显著高于 B组 ;D组的第 16周和第 2 8周都显著高于 B组。结论 :长期使用大剂量激素可以诱发正常大鼠发生肾小球损害 ,而用于大鼠阿霉素肾病模型则可加速肾小球损害的进程。
OBJECTIVE: To understand whether or not glucocorticoid (hormone) adrenoceptor aggravates glomerular damage in rats with adriamycin nephropathy. Methods: Forty male SD rats were randomly divided into normal control group (group A), doxorubicin group (group B), ADM and hormone group (group C) and hormone group (group D). Groups B and C were injected with doxorubicin 7mg / kg once through the tail vein. At the 16th week and the 28th week of the experiment, the kidneys and blood of 5 rats were taken from each group. The pathological changes of kidney were observed and the changes of urine protein, serum creatinine, triglyceride and total cholesterol were measured. Results: After 1 week of adriamycin injection, urinary protein began to rise, reaching 16 8.45 ± 76.28 mg / d after 4 weeks. After using hormone for 12 to 24 weeks (16 to 28 weeks after injection of doxorubicin), urine protein, serum creatinine, triglyceride and cholesterol in group B were significantly higher than those in group A Comparison, urinary protein, serum creatinine increased significantly, plasma albumin decreased significantly. In group C and group B, urinary protein, triglyceride and cholesterol increased significantly at the 16th week and 28th week, and serum creatinine increased significantly at the 16th week. Group D urine protein, triglyceride and cholesterol were significantly higher than the A group. The integral of pathological changes of kidney in group B was significantly higher than that in group A, and was significantly higher than that in week 16 in group 28; group C in group 16 was significantly higher than group B in week 16; group D was significantly higher in group 16 and 28 In group B CONCLUSION: Long-term use of high-dose hormones can induce glomerular damage in normal rats, whereas adriamycin-induced nephropathy in rats can accelerate the progression of glomerular damage.