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目的:观察肺岩宁方及其拆方对C57小鼠Lewis肺癌肿瘤组织中血管内皮生长因子(vascularendothelial growth factor,VEGF)及其激酶功能区受体(kinase-domain insert containing receptor,KDR)mRNA与蛋白表达的影响。方法:建立C57小鼠Lewis肺癌模型,随机分为模型组、肺岩宁组、益气组、补肾组和抗癌组,每组10只。分别给予相应药物干预,均灌胃21 d,第22天处死。观察肺转移情况,取肿瘤组织,采用逆转录聚合酶链反应(RT-PCR)法和免疫组化SP法分别检测C57小鼠Lewis肺癌中VEGF和KDR mRNA及蛋白表达水平。结果:肺岩宁组与补肾组、抗癌组小鼠的肺转移率明显低于模型组与益气组(P<0.05)。C57小鼠Lewis肺癌组织中表达VEGF和KDR,但肺岩宁组与补肾组、抗癌组小鼠肿瘤组织中VEGF mRNA及蛋白的表达水平均明显低于模型组和益气组(P<0.05),肺岩宁方组和抗癌组小鼠肿瘤组织中KDR mR-NA及蛋白的表达水平均明显低于模型组和益气组(P<0.05)。结论:肺岩宁抑制C57小鼠Lewis肿瘤转移的机制可能与下调VEGF及其受体KDR mRNA和蛋白表达有关。
Objective: To observe the effect of “Feiyanning Decoction” and its decomposed formulas on the expression of vascular endothelial growth factor (VEGF) and its kinase-domain insert containing receptor (KDR) in Lewis lung carcinoma C57 mice Effect of protein expression. Methods: Lewis lung cancer model of C57 mice was established and randomly divided into model group, Feiyanning group, Yiqi group, Bushen group and anti-cancer group, with 10 in each group. Respectively given the corresponding drug intervention, were intragastric administration of 21 d, the first 22 days were sacrificed. The lung metastasis was observed. Tumor tissues were taken out. The mRNA and protein expression of VEGF and KDR in C57 mice Lewis lung carcinoma were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (SP) respectively. Results: The lung metastasis rate in Feiyanning group, Bushen group and anti-cancer group was significantly lower than that in model group and Yiqi group (P <0.05). VEGF and KDR were expressed in Lewis lung cancer tissues of C57 mice, but the expression levels of VEGF mRNA and protein in the tumor tissues of Feiyanning and Bushen groups were significantly lower than those in model group and Yiqi group (P <0.05) ), KDR mR-NA and protein expression in the lung cancer Ning Fang group and anti-cancer mice were significantly lower than the model group and the Yiqi group (P <0.05). CONCLUSION: The mechanism of Feiyanning in inhibiting Lewis tumor metastasis in C57 mice may be related to the down-regulation of KDR mRNA and protein expression of VEGF and its receptor.