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目的观察重组小鼠β防御素3(r MBD3)对甲型流感病毒感染小鼠的保护作用。方法以10 mg·kg-1·d-1r MBD3腹腔注射3月,观察小鼠的一般状态,以及对主要脏器的影响。BALB/c小鼠,♀,分为正常对照组、模型对照组、r MBD3高剂量组(10 mg·kg-1·d-1)、r MBD3低剂量组(5 mg·kg-1·d-1)和利巴韦林组(100 mg·kg-1·d-1)。采用流感病毒液滴鼻感染建立甲型流感病毒H1N1感染小鼠模型,感染病毒12 h后分别腹腔注射给药。每日观察小鼠一般情况和死亡情况。于攻毒第3天检测肺泡灌洗液流感病毒滴度、血清γ干扰素含量、肺指数和肺组织病理变化。结果小鼠腹腔注射10 mg·kg-1·d-1r MBD3 3月,未发现明显异常反应或死亡,各重要脏器的未见明显病理改变。r MBD3各剂量组肺泡灌洗液中的病毒滴度、肺指数抑制率均降低,肺组织病理改变减少。结论 r MBD3对小鼠无明显毒性作用,在体内具有抗流感病毒、保护流感小鼠的活性。
Objective To observe the protective effect of recombinant mouse β defensin 3 (r MBD3) on influenza A virus-infected mice. Methods MBD3 was administered intraperitoneally at a dose of 10 mg · kg-1 · d-1r for 3 months to observe the general status of the mice and the effects on major organs. BALB / c mice were divided into normal control group, model control group, r MBD3 high dose group (10 mg · kg -1 · d -1), r MBD 3 low dose group (5 mg · kg -1 · d -1) and ribavirin group (100 mg · kg-1 · d-1). The mouse model of influenza A virus H1N1 infection was established by nasal infection of influenza virus and was administered intraperitoneally after 12 h of infection. Daily observation of the general situation and death of mice. On the third day of challenge, the titers of BALF, serum IFN-γ, lung index and pathological changes of lung tissue were detected. Results Mice were injected intraperitoneally with 10 mg · kg-1 · d-1r MBD3 for 3 months. No significant abnormal reaction or death was found. No significant pathological changes were observed in all the major organs. r MBD3 in each dose group in the lavage fluid in the virus titer, lung index inhibition rate decreased lung pathological changes decreased. Conclusion r MBD3 has no toxic effect on mice, anti-influenza virus in the body and protect the activity of influenza mice.