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目的:研究PPAR-γ在非酒精性脂肪性肝病(NAFLD)大鼠肝脏组织中的表达,探讨非酒精脂肪性肝病可能的发病机制。方法:雄性SD大鼠30只,随机分为A(正常组)15只普通饮食,B(高脂组)15只高脂饮食。8周后,自两组各随机抽取2只大鼠处死,光镜观察证实脂肪肝造模成功,继续喂养4周后处死所有大鼠,取血清做免疫生化检查,取肝组织标本,分别以光镜观察做出NAS评分,免疫组化和PCR法检测肝组织PPAR-γ蛋白的表达。结果:1.高脂饮食可以成功的复制NAFLD的大鼠模型;2.血清GLU、TC、TG、HDL-C、LDL-C在高脂组表达量较正常组明显升高,差异具有统计学意义(P<0.05);3.免疫组化显示:高脂组PPAR-γ表达量较正常组升高;结论:1.高脂饮食可成功复制NAFLD模型;2.PPAR-γ在NAFLD大鼠肝脏成脂性改变中具有重要作用。
Objective: To investigate the expression of PPAR-γ in liver tissues of rats with non-alcoholic fatty liver disease (NAFLD) and to explore the possible pathogenesis of non-alcoholic fatty liver disease. Methods: Thirty male SD rats were randomly divided into A (normal group) 15 normal diet and B (high fat group) 15 high fat diet. After 8 weeks, two rats were randomly selected from each of the two groups. The rats were sacrificed by light microscopy. Fatty liver was successfully established by light microscopy. All rats were sacrificed after 4 weeks of continuous feeding. Immuno-biochemical tests were performed on serum samples taken from liver tissues, NAS score was made by light microscope. The expression of PPAR-γ protein in liver tissue was detected by immunohistochemistry and PCR. High-fat diet can successfully replicate the NAFLD rat model; 2. Serum GLU, TC, TG, HDL-C, LDL-C expression in the high-fat group was significantly higher than the normal group, the difference was statistically significant (P <0.05) .3. Immunohistochemistry showed that the expression of PPAR-γ in hyperlipidemia group was higher than that in normal group.Conclusion: 1.High fat diet can successfully replicate NAFLD model.2.PPAR- The liver plays an important role in adipogenic changes.