论文部分内容阅读
目的 建立可移植高转移性兔原位膀胱癌模型。方法 VX 2细胞株采用肿块包埋法、细胞悬液注射法移植于新西兰兔膀胱。分别于 1、2、3周后处死动物及 5～ 6周荷瘤兔死亡后行病理检查移植瘤生长情况及淋巴结、肝、双肺转移情况。结果 两种方法接种成功率均为 1 0 0 %。1周后肿瘤大小为 0 .5cm ,2周为 1 .0～ 2 .0cm ,3周后膀胱壁全程浸润 ,同时出现广泛淋巴结及双肺转移。细胞悬液注射法组兔的肿瘤生长及转移速度慢于肿块包埋法组。结论 VX 2细胞株制成家兔原位膀胱癌具有种植成功率高、高转移性特点 ,其生物学特性与高度恶性的膀胱肿瘤一致。
Objective To establish a transplantable high metastatic rabbit orthotopic bladder cancer model. Methods The VX 2 cell line was transplanted into the bladder of New Zealand rabbits by means of tumor embedding and cell suspension injection. Animals were sacrificed 1, 2, and 3 weeks later and tumor-bearing rabbits were sacrificed 5 to 6 weeks after transplantation. The growth of transplanted tumors and the metastasis of lymph nodes, liver, and lungs were examined by pathology. Results The success rate of both methods was 100%. After 1 week, the tumor size was 0.5 cm, and 2 weeks was 1.0 to 2.0 cm. After 3 weeks, the bladder wall was infiltrated. At the same time, extensive lymph nodes and lung metastases occurred. The tumor growth and metastasis of rabbits in the cell suspension injection group were slower than those in the tumor-embedded group. Conclusions Rabbits with orthotopic bladder cancer produced by VX2 cell line have the characteristics of high success rate and high metastasis, and their biological characteristics are consistent with highly malignant bladder tumors.