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应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。HBV DNA在慢性活动性肝炎中检出率最高(80.7％),显著高于肝硬化、慢性小叶性肝炎、急性肝炎及原发性肝癌组。具有乙肝复制指标阳性患者的肝细胞内HBV DNA检出率(82.0％)明显高于非复制组(63.0％),P<0.05。本研究观察到;HBB DNA阳性肝细胞与肝细胞坏死灶关系密切,多位于肝细胞坏死灶中间或(和)周边,其中以局灶型分布的HBVDNA阳性肝细胞与肝细胞坏死灶关系密切。提示HBV复制与肝细胞坏死有关。
Biotinylated HBV DNA (HBV DNA) was used as a probe to study the in situ hybridization of 129 liver tissues from patients with liver disease. The detection rate of HBV DNA was the highest in chronic active hepatitis (80.7%), which was significantly higher than that of cirrhosis, chronic lobular hepatitis, acute hepatitis and primary liver cancer. The detection rate of HBV DNA in hepatocytes with positive hepatitis B (82.0%) was significantly higher than that in non-replication (63.0%), P <0.05. This study observed; HBB DNA positive hepatocytes and liver cell necrosis is closely related, and more in the middle of liver cell necrosis or (and) around, including focal distribution of HBVDNA positive hepatocytes and liver cell necrosis is closely related. Tip HBV replication and liver cell necrosis related.