Reported values(0.2 MPa-7.0 GPa) of the interlayer shear strength(ISS) of graphite are very dispersed.The main challenge to obtain a reliable value of the ISS using conventional measuring methods was the unavailability of sufficiently large single crystalline graphite.Here we present a novel experimental method to measure the ISS,and obtain the value as ～0.14 GPa.Our result can serve as an important basis for understanding mechanical behavior of graphite or graphene-based materials. Reported values ​​(0.2 MPa-7.0 GPa) of the interlayer shear strength (ISS) of graphite are very dispersed. The main challenge to obtain a reliable value of the ISS using conventional measuring methods was the unavailability of sufficiently large single crystalline graphite. Here we present a novel experimental method to measure the ISS, and obtain the value as ~ 0.14 GPa.Our result can serve as an important basis for understanding mechanical behavior of graphite or graphene-based materials.