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作者用可生物降解的丙交酯-乙交酯共聚体(DL-PLG)微粒包载由重组结核杆菌38kDa蛋白(免疫原性最强的抗原之一),构成结核菌苗传递系统(MP-38),并用同量抗原乳化于不完全弗氏佐剂制成IFA-38,对两者诱导的细胞及体液免疫效果进行比较。将MP-38和IFA-38分别于皮下、腹腔和胁腹免疫C57BL/10小鼠。皮下组于免后8天取腘窝淋巴结(LN)制成单细胞悬液,用重组38kDa抗原和合成肽进行T淋巴细胞增殖测定,用双夹心ELISA法检测细胞因子;腹腔组在免后3、5、7周采血,以ELISA法测总抗体和IgG1、IgG2a;胁腹组于免疫4～6周后静脉注射10~6个活结核杆菌H37Rv株,活菌感染后3～7周取脾计算菌落形成单位(CFU)。
The authors encapsulated the recombinant Mycobacterium tuberculosis 38 kDa protein, one of the most immunogenic, with biodegradable DL-PLG microparticles to construct the tuberculosis vaccine delivery system (MP- 38), and IFA-38 was made by emulsifying the same amount of antigen in incomplete Freund’s adjuvant to compare the cellular and humoral immune effects induced by both. C57BL / 10 mice were immunized subcutaneously, intraperitoneally and abdomen with MP-38 and IFA-38, respectively. The hypodermic group was taken from the popliteal lymph node (LN) for 8 days to make a single cell suspension. T lymphocyte proliferation was measured by recombinant 38 kDa antigen and synthetic peptide. Cytokines were detected by double sandwich ELISA. , 5,7 weeks of blood collection to measure the total antibody by ELISA and IgG1, IgG2a; flank group after immunization 4 to 6 weeks after intravenous injection of 10 to 6 Mycobacterium tuberculosis H37Rv strain live 3 to 7 weeks after infection spleen Colony forming units (CFU) were calculated.