论文部分内容阅读
目的:研究蛇葡萄素钠对人肺腺癌SPC-A-1细胞、人正常胚肺MRC-5细胞的毒性作用。方法:应用MTT法检测细胞增殖,流式细胞仪检测细胞周期和细胞凋亡;透射电子显微镜(TEM)观察细胞超微结构的变化。结果:MTT法实验表明,蛇葡萄素钠、卡铂显著抑制SPC-A-1细胞的增殖,呈现浓度依赖性,IC50分别为57.18±9.42 ug/ml、33.56±11.2 ug/ml,但对MRC-5细胞增殖的抑制作用不明显,IC50分别大于5000 ug/ml及500ug/ml;流式细胞检测分析表明,25~100ug/ml的蛇葡萄素钠可使SPC-A-1细胞增殖阻滞于S期,G0/G1期细胞明显减少,且呈浓度依赖性,50、100 ug/ml的蛇葡萄素钠可使SPC-A-1细胞发生明显凋亡,而蛇葡萄素钠对MRC-5细胞周期及凋亡的影响不明显。蛇葡萄素钠浓度为50、100ug/ml时,透射电镜下可见SPC-A-1细胞表现出典型的凋亡特征,而MRC-5细胞变化不明显。结论:蛇葡萄素钠对肿瘤细胞具有较强的毒作用,对正常细胞毒性较小。
OBJECTIVE: To study the toxic effects of snake venote sodium on human lung adenocarcinoma SPC-A-1 cells and human normal embryo lung MRC-5 cells. Methods: Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were detected by flow cytometry. Transmission electron microscopy (TEM) was used to observe the changes of cell ultrastructure. Results: The results of MTT assay showed that sodium phytocerebroside and carboplatin significantly inhibited the proliferation of SPC-A-1 cells in a concentration-dependent manner with IC50 of 57.18 ± 9.42 ug / ml and 33.56 ± 11.2 ug / ml, respectively. However, The results of flow cytometry showed that 25 ~ 100ug / ml of sodium hypophosphite could inhibit the proliferation of SPC-A-1 cells In S phase, the cells in G0 / G1 phase decreased significantly, and in a concentration-dependent manner, 50 and 100 μg / ml of sodium hypophosphite could induce significant apoptosis in SPC-A-1 cells, 5 cell cycle and the impact of apoptosis is not obvious. SPC-A-1 cells showed typical apoptotic characteristics under transmission electron microscopy, but the changes of MRC-5 cells were not obvious. Conclusion: Sodium Sulphate sodium has strong toxic effect on tumor cells and less cytotoxicity to normal cells.