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目的:探讨玄参化学成分的抗氧化作用.方法:应用多种层析方法和化学和光谱方法,对玄参的水溶性主要成分进行分离和结构鉴定.应用脉冲辐解方法,观察分离得到的主要单体成分对脱氧核苷酸dAMP和dGMP羟基加成自由基的快速修复过程,测定苯丙素苷与羟基加成自由基的反应速率常数.结果:从玄参水溶性部位分得四种主要成分,发现它们属于环烯醚萜苷:哈帕酯苷与哈帕苷,和苯丙素苷:安格洛苷C与acteoside.脉冲辐解实验中观察到在0.1 mmol·L~(-1)时,苯丙素苷安格洛苷C与acteoside对脱氧核苷酸羟基加成自由基产生显著的修复作用,而环烯醚萜苷:哈帕酯苷与哈帕苷在相同条件下作用不明显.安格洛苷C与dAMP及dGMP间的电子转移速率常数为4.2×10~8及10.3×10~8 L·mol~(-1)·S~(-1);acteoside与dAMP及dGMP间的电子转移速率常数为5.3×10~8及20.2×10~8 L·mol~(-1)·S~(-1).结论:玄参中的苯丙素苷在还原脱氧核苷酸的氧化性羟基加成自由基方面有很好的抗氧化作用.
Objective: To investigate the anti-oxidation effect of chemical constituents of Radix Scrophulariae. Methods: A variety of chromatographic methods and chemical and spectroscopic methods were used to separate and identify the main water-soluble constituents of Radix Scrophulariae. The pulsed radiolysis method was used to observe the separation. The rapid recovery of the deoxynucleotide dAMP and dGMP hydroxyaddition free radicals of the main monomer component was determined by measuring the reaction rate constant between the phenylpropanoid glycoside and the hydroxyaddition free radical. Results: Four species were obtained from the water-soluble part of Scrophulariae. The main constituents were found to be iridoid glycosides: halpaside and lappaside, and phenylpropanoid glycosides: Anguloside C and acteoside. Pulse radiolysis experiments were observed at 0.1 mmol·L~(- 1) Phenylpropanoid Anguloside C and acteoside produce significant repair of deoxynucleotide hydroxyaddition free radicals, while iridoid glycosides: harpapaside and lappaside under the same conditions The effect is not obvious. The electron transfer rate constants between Anglioside C and dAMP and dGMP are 4.2×10~8 and 10.3×10~8 L·mol~(-1)·S~(-1); acteoside and dAMP The electron transfer rate constants between dGMP and dGMP were 5.3×10~8 and 20.2×10~8 L·mol~(-1)·S~(-1). Conclusion: The phenylpropanoid glycosides in Scrophulariae were reduced. The deoxynucleotide has good antioxidant effect on the oxidative hydroxyl radicals.