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为了寻找与肝细胞癌转移相关的蛋白,本研究选择了具有相同遗传背景但不同转移能力的两个肝癌细胞系MHCC97H和HCCLM6,在采用超滤辅助样品制备(filter aided proteome preparation,FASP)方法和相对和绝对定量同位素标记(isobaric tags for relative and absolute quantitation,i TRAQ)技术对样品进行处理后,对所提取的蛋白质进行了质谱鉴定和定量分析,对差异蛋白进行了包括亚细胞定位,生物学过程和分子功能富集在内的生物信息学分析。我们共鉴定了4 987个蛋白,与MHCC97H细胞系相比HCCLM6中有93个差异表达蛋白(|ratio|≥1.5,一个样本t检验,p<0.05),其中40个蛋白上调,53个蛋白下调,且大多数差异表达的蛋白质位于细胞质和细胞膜。GO分析表明与细胞粘附和蛋白质结合相关的蛋白质在差异蛋白中显著富集。本研究为肝癌转移机制的进一步研究提供了一定的帮助。
In order to find out the proteins involved in the metastasis of hepatocellular carcinoma, two hepatocarcinoma cell lines, MHCC97H and HCCLM6, with the same genetic background but different metastatic potential, were selected for this study. In this study, a filter aided proteome preparation (FASP) After the samples were treated by isobaric tags for relative and absolute quantitation (i TRAQ), the extracted proteins were identified by mass spectrometry and quantified. The differential proteins were characterized by subcellular localization, biology Bioinformatics Analysis of Process and Molecular Function Enrichment. We identified a total of 4 987 proteins with 93 differentially expressed proteins (| ratio | ≧ 1.5, one sample t-test, p <0.05) in HCCLM6 compared to the MHCC97H cell line, of which 40 were up-regulated and 53 were down-regulated , And most differentially expressed proteins are located in the cytoplasm and cell membrane. GO analysis showed that proteins associated with cell adhesion and protein binding were significantly enriched in differential proteins. This study provides some help for the further study of liver cancer metastasis mechanism.