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本实验用聚丙烯酰胺凝胶电泳法分析番茄四个高优组合和一个低优组合芽期过氧化物酶同工酶的变化情况。按生长顺序分Ⅵ次取样。通过酶谱技术显示谱带图样说明,在芽期随生长的进展,过氧化物酶同工酶有明显变化。在高优组合中,亲本酶活性从第Ⅰ次取样到第Ⅴ次取样酶活性逐渐增加,第Ⅵ次取样酶活性则比第Ⅴ次下降;而高优组合F_1从第Ⅰ到第Ⅵ次取样,酶活性表现为一直在增加。F_1酶谱带与亲本酶谱带有明显差异,但未发现有“杂种带”。在低优组合中,酶活性较低;F_1酶谱带与母本相同。把高优组合的两个亲本取样Ⅵ的扫描图绘在同一座标中,可有多点交叉,而低优组合没有或很少交叉;高优组合F_1的酶活性总面积大于两个亲本,而低优组合F_1的酶活性总面积则小于两个亲本。由此说明,番茄芽期过氧化物酶同二酶的变化与杂种优势有密切关系,分析同工酶的变化规律,可为予测杂种优势,探讨杂种优势形成机理提供依据。
In this experiment, polyacrylamide gel electrophoresis analysis of four high-yielding tomato combinations and a low-yield combinations of peroxidase isoenzyme changes. Divided by the order of growth Ⅵ sampling. By zymogram showed that the band pattern shows that at the bud stage with the progress of growth, peroxidase isozymes have significant changes. In the high-yielding combination, the activity of the parent enzyme increased from the first sampling to the fifth sampling, while the activity of the fifth sampling decreased compared with the fifth. The high-quality combination F_1 was sampled from the first to the Ⅵ , Enzyme activity has been shown to have been increasing. There was a significant difference between the F_1 enzyme band and the parental zymogram, but no “hybrid band” was found. The enzyme activity was lower in the lower optimal combinations; the F_1 enzyme bands were the same as the female parent. The high-quality combination of the two parent sample VI scan was plotted in the same coordinates, there may be more crossover, and low-excellent combination of little or no crossover; high-excellent combination of F 1 enzyme activity of the total area of more than two parents, However, the total area of low activity F_1 was less than that of two parents. Thus, the changes of the same dipeptide enzyme and the heterosis in the budding stage of tomato were closely related to the analysis of the variation of isozymes, which could provide basis for the prediction of heterosis and the formation mechanism of heterosis.