用溶液进行分光光度法的定量测定已沿用了一个世纪,自从有了可靠的可调谐染料激光器,出现了一系列崭新的测量吸光度的方法,例如激光腔内吸收、热透镜、光声光谱方法等,这些方法使分光光度法的灵敏度及检测下限发生了飞跃。虽然这些高灵敏度的方法,在国外已作过许多深入研究,但是在国内该类工作报导的不多。由于可调谐染料激光器的稳定性不够,虽然有了很高的吸光度,但是可用的测定下限受 Quantitative determination of the spectrophotometric method with solution has been around for over a century, and since reliable tunable dye lasers have been introduced a number of new methods have been developed for measuring absorbance such as laser cavity absorption, thermal lensing, photoacoustic spectroscopy, etc. , These methods have led to a leap in the sensitivity and detection limit of spectrophotometry. Although these highly sensitive methods have undergone many in-depth researches in foreign countries, there are not many reports of such work in China. Due to the lack of stability of tunable dye lasers, the lower limit of determination that is available, despite the high absorbance