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目的 :建立常规的 PCR- SSP基因分型方法。方法 :对 16名患者及其家系 HL A- DR进行血清学和 PCR- SSP分型。结果 :血清学分型 7对供、受体相合 ,而 PCR- SSP基因分型只有其中 5对相合 ,另外 2对 1对为错判、1对为 DR2两个亚型 DR15、DR16间的错判。结论 :PCR- SSP基因分型是一种快速、准确和可行的 HLA分型方法 ,具有广阔的临床应用前景。
Objective: To establish a routine PCR-SSP genotyping method. Methods: Serological and PCR-SSP typing was performed on 16 patients and their family HL A-DR. RESULTS: Serology type 7 matched the donor and acceptor, but PCR-SSP genotyping only had 5 pairs of matched, and 2 pairs of 1 pair were misjudged, 1 pair was DR2, and the two subtypes DR15 and DR16 were misjudged. . Conclusion : PCR-SSP genotyping is a rapid, accurate and feasible HLA typing method, and has broad clinical application prospects.