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目的:研究实验性单侧前牙反(unilateral anterior crossbite,UAC)对大鼠髁突软骨的致病意义及对促软骨细胞分化的CaMKⅡ表达的影响。方法:40只6周龄SD雌性大鼠,随机等分为UAC组和对照组,其中UAC组左侧上下切牙各戴一套筒冠并形成反关系,对照组不作处理。于实验开始后4周和8周取材,HE和番红O染色观察髁突软骨的组织形态变化,免疫组织化学染色观察CaMKⅡ的表达,Real-time PCR测量相关基因表达水平的变化。结果:UAC组髁突软骨细胞分化异常,软骨变薄,基质减少,且随时间的延长而加重(P<0.05),CaMKⅡ阳性细胞百分数以及CaMKⅡ和Mmp-13的mRNA表达水平上调,但促增殖的Pcna以及软骨基质Col2a1、Aggrecan的mRNA的表达水平下调。结论:UAC可导致髁突软骨异常分化并伴有CaMKⅡ的高表达。
Objective: To investigate the pathogenic significance of experimental unilateral anterior crossbite (UAC) on rat condylar cartilage and its effect on the expression of CaMKⅡ induced by chondrocytes. Methods: Forty SD female rats of 6 weeks old were randomly divided into UAC group and control group. The upper and lower incisors on the left side of UAC group were fitted with a set of canopies and formed an inverted relationship. The control group was not treated. At 4 weeks and 8 weeks after the start of the experiment, the morphological changes of condylar cartilage were observed by HE and Safranin O staining. The expression of CaMKII was observed by immunohistochemical staining. The expression of related genes was measured by Real-time PCR. RESULTS: In the UAC group, the condylar chondrocytes were abnormally differentiated, the cartilage became thinner, the matrix was decreased, and the expression of CaMKⅡ and Mmp-13 mRNA was up-regulated in the UAC group (P <0.05) Of Pcna and cartilage matrix Col2a1, Aggrecan mRNA expression levels were down-regulated. Conclusion: UAC can lead to the abnormal differentiation of condylar cartilage accompanied with the high expression of CaMK Ⅱ.