论文部分内容阅读
目的:通过主要催眠药效研究对参苓颗粒提取纯化工艺进行筛选,为确定该制剂的制备工艺提供实验依据。方法:自制单提油醇沉、单提油不醇沉、合提油醇沉及合提油不醇沉4组不同工艺路线的参苓颗粒浸膏,采用翻正反射睡眠试验考察各组浸膏对小鼠阈下、阈剂量戊巴比妥钠协同作用的影响;采用环磷酰胺、氢化可的松致阴血亏虚及水平台法剥夺睡眠48 h,建立阴血亏虚失眠证候大鼠复合模型,通过HPLC测定各组浸膏大鼠脑组织中氨基酸类神经递质谷氨酸(Glu),甘氨酸(Gly),牛磺酸(Tau)及γ-氨基丁酸(GABA)的含量变化。结果:4组浸膏对小鼠阈下、阈剂量戊巴比妥钠均表现不同程度的协同作用,尤以单提油醇沉组显著缩短小鼠睡眠潜伏期、显著延长小鼠睡眠时间;与空白组比较,模型组大鼠脑组织Glu,Tau及GABA含量均明显升高,Glu/(Tau+GABA)比例增高,经各浸膏组干预后含量均不同程度下降,其中单提油醇沉组表现更为显著,Glu/(Tau+GABA)比例也在下调。结论:参苓颗粒的制备工艺优选单独提取挥发油合并醇沉的纯化工艺路线。
OBJECTIVE: To screen and purify the extraction and purification of Shenling granules by the main hypnotic efficacy study, and provide experimental evidence for the preparation of the preparation. Methods: The self-made single mention alcohol precipitation, single mention of non-alcohol precipitation, combined with alcohol precipitation and non-alcohol Shen Shen 4 groups of different routes Shenling granules extract, the use of regular reflex sleep test study groups of dip Cream on the mouse subthreshold and threshold doses of pentobarbital sodium synergies; cyclophosphamide, hydrocortisone-induced hypoxemia and water level deprivation of sleep 48 h, the establishment of Yin and blood deficiency insomnia syndrome rats Compound model, the changes of amino acid neurotransmitters glutamate (Glu), glycine (Gly), taurine (Tau) and γ-aminobutyric acid (GABA) . Results: The 4 groups of extracts had synergistic effects on the subthreshold and threshold dose of pentobarbital sodium in mice, especially the single-dose oleyl alcohol group significantly shortened the sleep latency and prolonged the sleep time in mice; Compared with blank group, the content of Glu, Tau and GABA in brain tissue of model group were significantly increased, while the ratio of Glu / (Tau + GABA) increased. The content of Glu / Tau + GABA decreased in different degree after intervention, Group showed more significant, Glu / (Tau + GABA) ratio is also down. Conclusion: The preparation process of Shenling granules is the best one to separate the purification process of volatile oil combined with alcohol precipitation.