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稻粒黑粉病(Rice kernel smut)是一种真菌病害。在离体条件下,采用孢子萌发测定法,测定了嘧菌酯、灭菌唑、拌种灵、多菌灵、咪酰胺和二硫氰基甲烷抑制稻粒黑粉病菌冬孢子萌发的最低抑制浓度(MIC);同时研究了药剂浸种处理对稻种的安全性以及浸种处理效果。结果表明,嘧菌酯和二硫氰基甲烷对稻粒黑粉病菌冬孢子萌发的抑制活性较高,MIC为0.4μg/mL,多菌灵的抑制活性次之,MIC为0.8μg/mL,其他3种药剂的抑制活性不高,MIC均大于1.6μg/mL。种子安全性试验表明,嘧菌酯在2.0、4.0、8.0μg/mL和多菌灵在4.0、8.0、16.0μg/mL处理3d和7d后,稻种的萌芽率与对照相比无显著性差异;而二硫氰基甲烷在4.0和8.0μg/mL处理下,所选稻种的萌芽率显著低于对照,说明该药剂在浸种处理后会影响所选稻种的萌芽,但其在低剂量处理下对稻种的萌芽率影响较小。关于稻粒黑粉病浸种处理的合适农药剂型仍需要进一步研究。
Rice kernel smut is a fungal disease. In vitro, spore germination assay was used to determine the minimum inhibitory effect of azoxystrobin, azoxystrobin, seed dressing, carbendazim, picoline and dithiocyanomethane on the inhibition of teliospore germination Concentration (MIC). At the same time, the safety of seed soaking with chemicals and the effect of seed soaking treatment were studied. The results showed that the inhibitory activity of azoxystrobin and dithiocyanomethane on teliospore germination of S. smithii was high, with a MIC of 0.4 μg / mL and followed by carbendazim with a MIC of 0.8 μg / mL, The inhibitory activity of the other three agents is not high, MIC were greater than 1.6μg / mL. Seed safety tests showed that there was no significant difference in the germination rate of azoxystrobin between 2.0, 4.0 and 8.0 μg / mL azoxystrobin and carbendazim at 4.0, 8.0 and 16.0 μg / mL for three and seven days ; While under the treatment of 4.0 and 8.0 μg / mL of dithiocyanomethane, the germination rate of selected rice seeds was significantly lower than that of the control, indicating that the agent affected seed germination of the selected rice seeds after soaking, but at the low dose Under the treatment of rice seed germination rate less affected. There is still room for further research on the suitable pesticide formulations for the treatment of rice seed powder soaking.