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郎格罕细胞（ＬＣ）在角膜排斥反应中起着重要的作用，若能在角膜移植前后选择性地抑制ＬＣ的功能活性，有可能延长移植角膜的存活期。本实验选用免疫抑制剂──氢化可的松处理小鼠眼球，利用酶组化──ＡＴＰ酶法和图像分析仪，观察其对角膜、结膜上皮内ＬＣ密度以及酶活性的影响。结果显示，随着氢化可的松浓度的增大，ＬＣ内ＡＴＰ酶反应程度、ＬＣ密度显示下降趋势。当氢化可的松浓度增加至１．７ｍｇ／ｍｌ时，除角巩缘外，周边角膜和结膜已无ＡＴＰａｓｅ阳性的ＬＣ，当浓度达到２．０ｍｇ／ｍｌ时，角巩缘也很少见ＡＴＰａｓｅ阳性的Ｌｃ。
Langerhans cells (LC) play an important role in corneal rejection, and if the functional activity of LC is selectively inhibited before and after corneal transplantation, it is possible to prolong the survival of the cornea. In this experiment, the immunosuppressant - hydrocortisone mouse eye treatment, the use of enzyme histochemistry - ATP enzymatic and image analyzer to observe the cornea, conjunctiva epithelial LC density and enzyme activity. The results showed that with the increase of hydrocortisone concentration, LC within ATP enzyme reaction, LC density showed a downward trend. When the hydrocortisone concentration increased to 1.7mg / ml, in addition to the angle of the margins of the cornea and conjunctiva have no ATPase-positive LC, when the concentration reached 2.0mg / ml, the angle of the margins are also rare ATPase Positive Lc.