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AIM:To establish nested-PCR methods for the detectionof SENV-D and SENV-H and to investigate the epidemiologyof SEN virus in China.METHODS:According to published gene sequences,primers from the conserved region were designed.Then,135 samples from healthy voluntary blood donors and 242samples from patients with various forms of liver diseasewere detected by nested-PCR of SENV-D/H.Some PCRproducts were cloned and sequenced.RESULTS:By sequencing,the specificity of genotype-specific PCR was confirmed.SENV-D/H DNA was detectedin 31% of the blood donors,which was higher than thosein America and Italy (2%),and in Japan and Taiwan (15-20%).The prevalence of SENV-D/H viremia was significantlyhigher in patients with hepatitis B and hepatitis C than inblood donors (59-85% vs31%,P<0.05).The prevalenceamong patients with non-A-E hepatitis was significantlyhigher than among blood donors (68% vs 31%,P<0.01),and equivalent to that among patients with hepatitis B and C.CONCLUSION:Nested-PCR with genotype-specific primerscould serve as a useful SENV screening assay.SENV hasthe same transmission modes as HBV and HCV.The highprevalence in patients with non-A-E hepatitis may attributeto the transmission modes,and SENV may not serve asthe causative agents.
AIM: To establish nested-PCR methods for the detection of SENV-D and SENV-H and to investigate the epidemiology of SEN virus in China. METHODS: According to published conserved regions were primers. 135, samples from healthy voluntary blood donors and 242samples from patients with various forms of liver disease were detected by nested-PCR of SENV-D / H.Some PCR products were cloned and sequenced .RESULTS: By sequencing, the specificity of genotype-specific PCR was confirmed. SENV-D / H DNA was detected in 31% of the blood donors, which was higher than those in America and Italy (2%), and in Japan and Taiwan (15-20%). The prevalence of SENV-D / Hviremia was significantlyhigher in patients (59-85% vs 31%, P <0.05). The prevalence of patients with non-AE hepatitis was significantlyhigher than among blood donors (68% vs 31%, P <0.01), and equivalent to that among patients with hepatitis B and C.CONCLUSION: Nested-PCR with genoty pe-specific primers can serve as a useful SENV screening assay. SENV hasthe same transmission modes as HBV and HCV. the high prevalence in patients with non-A-E hepatitis may attribute to the transmission modes, and SENV may not serve asthe causative agents.