为观察痹康灵对骨关节炎 (OA)的治疗效果并探讨其作用机制 ,将 32只中国白兔分为正常对照组 (A组 )、空白对照组 (B组 )、西药对照组 (C组 )、中药治疗组 (D组 ) ,每组各 8只 ,A组不造模 ,B、C、D组均采用Hulth法建立兔OA模型。术后 3天给予A和B组生理盐水 ,C组罗非昔布溶液 ,D组痹康灵 ,每天各 6ml·kg-1,灌胃给药。自术后第 1周每天驱赶动物 2次 ,共 30分钟 ,4周后取右侧胫骨平台关节软骨和滑膜进行光镜观察。采用免疫组化检测法分别检测关节软骨和滑膜中的白细胞介素 1β(IL β)、基质金属蛋白酶 1(MMP 1)、基质金属蛋白酶 3(MMP 3)的水平。结果显示 ,B组IL 1β ,MMP 1、MMP 3的水平均非常显著高于A组(P <0 .0 1) ,并均显著高于C组 (0 .0 1

0 .0 5 )。光镜观察 ,B组退变较其余 3组明显 ;C组与D组间退变不明显 ;但 2组退变均甚于A组。表明痹康灵能抑制IL 1β、MMP 1、MMP 3的产生 ,具有抗炎、保护和修复关节软骨的作用 ,从而对OA有一定的治疗作用 To observe the therapeutic effect of Ji Kangling on osteoarthritis (OA) and explore its mechanism of action, 32 Chinese white rabbits were divided into normal control group (group A), blank control group (group B), and Western medicine control group (C Group) and traditional Chinese medicine treatment group (D group), 8 in each group, group A did not make model, group B, C and D all used Hulth method to establish rabbit OA model. Three days after surgery, the rats in group A and group B were given normal saline, group C was treated with rofecoxib, group D was given and each group was treated with 6 ml·kg-1 of glucagon per day. The animals were dislodged twice a day for 30 minutes from the first postoperative week. Four weeks later, the right tibial plateau cartilage and synovial membrane were taken for light microscopy. The levels of interleukin 1β (IL β), matrix metalloproteinase 1 (MMP 1) and matrix metalloproteinase 3 (MMP 3) in the articular cartilage and synovium were detected by immunohistochemistry. The results showed that the levels of IL 1β, MMP 1 and MMP 3 in group B were significantly higher than those in group A (P < 0.01) and were significantly higher than those in group C (0. 0 1

0.05). Observed by light microscope, the degeneration of group B was more obvious than that of the other three groups; the degeneration between group C and group D was not obvious; however, the degeneration of group 2 was more than that of group A. It shows that Jikangling can inhibit the production of IL 1β, MMP 1 and MMP 3, and it has the function of anti-inflammatory, protecting and repairing articular cartilage, thus having a certain therapeutic effect on OA.