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目的探讨肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)在尘肺患者不同阶段、不同类型血清中的表达水平及其意义。方法对70例尘肺患者,其中矽肺42例、煤工尘肺28例,壹期病例46例、贰期病例17例,叁期病例7例,以及40例同期健康检查者,抽取空腹静脉血5 ml,采用酶联免疫吸附测定(ELISA)法进行血清TNF-α和IL-6检测。结果尘肺组血清TNF-α水平(22.31±5.35)ng/L低于对照组(45.63±8.32)ng/L,差异有统计学意义(P<0.05);IL-6水平(18.78±2.46)ng/L高于对照组(10.74±3.71)ng/L,差异有统计学意义(P<0.05)。矽肺组和煤工尘肺组中随着期别的升高,血清TNF-α水平反而降低(P<0.05);而其血清IL-6水平升高(P<0.05)。结论 TNF-α和IL-6表达水平对于尘肺病的病情进展以及疗效观察分析有重要的意义,为尘肺病的预警和健康监护提供有意义的生物学指标。
Objective To investigate the expression and significance of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in different stages and types of pneumoconiosis patients. Methods Seventy patients with pneumoconiosis, including 42 cases of silicosis, 28 cases of coal worker’s pneumoconiosis, 46 cases of stage one, 17 cases of stage II, 7 cases of stage III and 40 cases of same period health examination, were given 5 ml fasting venous blood Serum TNF-α and IL-6 levels were detected by enzyme-linked immunosorbent assay (ELISA). Results The level of TNF-α in pneumoconiosis group was significantly lower than that in control group (22.31 ± 5.35 ng / L, 45.63 ± 8.32 ng / L, P <0.05), IL-6 level was 18.78 ± 2.46 / L was higher than that of the control group (10.74 ± 3.71) ng / L, the difference was statistically significant (P <0.05). The level of serum TNF-α decreased with the increase of stage in silicosis group and coal worker’s pneumoconiosis group (P <0.05), while the level of serum IL-6 increased (P <0.05). Conclusion The expression of TNF-α and IL-6 is of great significance for the progression of pneumoconiosis and the observation and analysis of the curative effect. It provides meaningful biological indicators for early warning and health monitoring of pneumoconiosis.